A brand new examine by the College of Barcelona has analysed the viability of a brand new nanomolecule as drug supply car. The outcomes, revealed within the journal Colloids and Surfaces B: Biointerfaces, present that liposomes designed by researchers are capable of transport and ship an anticancer drug that has been used as a mannequin contained in the cells. The examine included the participation of researchers from the College of Barcelona schools of Biology, Physics and Pharmacy and Meals Sciences, in addition to researchers from the Scientific and Technological Facilities of the UB (CCiTUB), the Institute of Nanoscience and Nanotechnology of the UB (IN2UB) and from the Institute for Bioengineering of Catalonia (IBEC).
Interplay between liposomes and the cell membrane
A liposome is a man-made spherical vesicle with a membrane made from a twin layer of lipids which has similarities to the construction of cell membranes. Since these molecules had been discovered, within the sixties, they’ve been used as a mannequin to review cell membranes and as a possible drug supply system.
One of many challenges of turning liposomes into drug supply automobiles is discovering out how they work together with cell membranes and what primary mechanism — adsorption, fusion or endocytosis, or a mix of those three — is concerned within the integration of liposomes by cells. “The interactions between liposomes and the cell membrane could be extraordinarily totally different relying on the character of the cell membrane and the lipidic composition of liposomes,” the researchers observe.
The liposomes designed by the UB workforce are small lipid spheres with a composition much like that of the cell they need to deal with. “This similarity eases its incorporation and the drug supply contained in the cell,” notes Òscar Domenech, IN2UB member and one of many researchers that took half within the examine.
Examine in cell cultures
This examine is the continuation of a earlier examine carried out by the identical analysis workforce which analysed the fusion mechanisms of liposomes utilizing a simplified mannequin that copied the membrane of HeLa cells, a kind of tradition cells that are broadly utilized in scientific analysis. “The membrane of HeLa cells is extra complicated than the mannequin we used within the earlier examine. Now, we have now used actual cell cultures with a purpose to acquire a greater view of the interplay mechanism of our liposomes,” the researchers say.
To review the interplay between the liposomes and the cell membrane and to evaluate the combination of those nanomolecules, the researchers mixed two strategies. On the one hand, they used confocal fluorescence, which permits them to see fluorescent molecules contained in the cell. Then, the liposomes encapsulated calcein, a fluorescent dye, to see whether or not the nanomolecule and its content material had been getting into the cells.
However, the researchers used the atomic power microscopy method to see the physicochemical adjustments of the cell floor and assess the stiffness of the cell membrane within the presence of liposomes. The interplay of the liposomes designed by the researchers affirmed the outcomes obtained with mannequin membranes and it reveals the capability of the formulation of those nanomolecules as a possible nanotransporter. “We present that the lipid composition allows a supply of the liposome content material contained in the cell, in addition to the impact of the filopodia — small flagella of the cell — in easing the arrival of liposomes on the cell membrane,” provides Òscar Domènech.
A check with an anticancer drug
To validate the capability of those liposomes as a drug supply system, the researchers encapsulated methotrexate, an immunosuppressive drug used within the remedy of a number of oncologic, inflammatory and autoimmune pathologies. “We might present that our liposomes are perfect for delivering this mannequin molecule, which we all know can eradicate most cancers cells,” notes Domènech.
These outcomes open the door to future research with different molecules and cell sorts. “Our curiosity could be to increase the methodology to different kinds of cells and even tissues to point out the viability of the evaluation, in addition to to make use of different therapeutical molecules encapsulated in liposomes,” says Domènech. “Furthermore — he provides — the 2 strategies we utilized through the examine allowed us to acquire ends in a quick and minimally invasive method, which sooner or later may very well be indicators of the great functioning of a drug in opposition to most cancers cells.”